Estimate Fatty Acid Calculation

To estimate the free fatty acid in feed.
For feed:
Weigh 20g of the feed and extract the oil from it using soxlet apparatus,then add 10ml of ethanol and mix properly,also add a drop of phenolpthalein and titre it against 0.143N sodium hydroxide.End point is the appearence of pale permanent pink colour.

Estimate Fatty Acid Calculator

  • Volume of sodium Hydroxide

  • Normality of sodium hydroxide

  • Equivalent factor

  • weight of sample

  • Percentage of free fatty acid


percentage of free fatty acid = ( V * N * F /weight of sample )

V - Volume of sodium hydroxide
N - Normality of sodium hydroxide
F - Equivalent factor
This online calculator helps you dynamically to calculate the percentage of free fatty acid easily.